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Cryo-electron microscopy reconstruction of PCAT1 bound to its CtA peptide substrate

ABSTRACT:

SUBMITTER: Jue Chen

PROVIDER: EMPIAR-10818 | biostudies-other |

REPOSITORIES: biostudies-other

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Structural basis of substrate recognition by a polypeptide processing and secretion transporter.

Kieuvongngam Virapat V Olinares Paul Dominic B PDB Palillo Anthony A Oldham Michael L ML Chait Brian T BT Chen Jue J

eLife 20200114

The peptidase-containing ATP-binding cassette transporters (PCATs) are unique members of the ABC transporter family that proteolytically process and export peptides and proteins. Each PCAT contains two peptidase domains that cleave off the secretion signal, two transmembrane domains forming a translocation pathway, and two nucleotide-binding domains that hydrolyze ATP. Previously the crystal structures of a PCAT from <i>Clostridium thermocellum</i> (PCAT1) were determined in the absence and pres ...[more]

PMID: 31934861

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