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Cryo-electron tomography of Cryo-FIB milled dividing E. coli envC and/or nlpD deletion strain.


ABSTRACT:

SUBMITTER: Paula Navarro 

PROVIDER: EMPIAR-11089 | biostudies-other |

REPOSITORIES: biostudies-other

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Publications

Cell wall synthesis and remodelling dynamics determine division site architecture and cell shape in Escherichia coli.

Navarro Paula P PP   Vettiger Andrea A   Ananda Virly Y VY   Llopis Paula Montero PM   Allolio Christoph C   Bernhardt Thomas G TG   Chao Luke H LH  

Nature microbiology 20220912 10


The bacterial division apparatus catalyses the synthesis and remodelling of septal peptidoglycan (sPG) to build the cell wall layer that fortifies the daughter cell poles. Understanding of this essential process has been limited by the lack of native three-dimensional views of developing septa. Here, we apply state-of-the-art cryogenic electron tomography (cryo-ET) and fluorescence microscopy to visualize the division site architecture and sPG biogenesis dynamics of the Gram-negative bacterium E  ...[more]

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