Dataset Information
Cryo-electron tomography of FIB-milled neuronal synapses in cultured primary neurons
Ontology highlight
ABSTRACT:
SUBMITTER: Richard Held
PROVIDER: EMPIAR-12109 | biostudies-other |
REPOSITORIES: biostudies-other
Publications
Nanoscale architecture of synaptic vesicles and scaffolding complexes revealed by cryo-electron tomography.
Proceedings of the National Academy of Sciences of the United States of America 20240626 27
The spatial distribution of proteins and their arrangement within the cellular ultrastructure regulates the opening of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors in response to glutamate release at the synapse. Fluorescence microscopy imaging revealed that the postsynaptic density (PSD) and scaffolding proteins in the presynaptic active zone (AZ) align across the synapse to form a trans-synaptic "nanocolumn," but the relation to synaptic vesicle release sites is uncert ...[more]