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CryoEM movies of extracted Arctic variant Aß42 fibrils from mouse brains labelled with methoxy-X04

ABSTRACT:

SUBMITTER: René AW Frank

PROVIDER: EMPIAR-11508 | biostudies-other |

REPOSITORIES: biostudies-other

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The in-tissue molecular architecture of β-amyloid pathology in the mammalian brain.

Leistner Conny C Wilkinson Martin M Burgess Ailidh A Lovatt Megan M Goodbody Stanley S Xu Yong Y Deuchars Susan S Radford Sheena E SE Ranson Neil A NA Frank René A W RAW

Nature communications 20230517 1

Amyloid plaques composed of Aβ fibrils are a hallmark of Alzheimer's disease (AD). However, the molecular architecture of amyloid plaques in the context of fresh mammalian brain tissue is unknown. Here, using cryogenic correlated light and electron tomography we report the in situ molecular architecture of Aβ fibrils in the App<sup>NL-G-F</sup> familial AD mouse model containing the Arctic mutation and an atomic model of ex vivo purified Arctic Aβ fibrils. We show that in-tissue Aβ fibrils are a ...[more]

PMID: 37198197

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