Ontology highlight
ABSTRACT:
SUBMITTER: Schwenen LL
PROVIDER: S-EPMC4499801 | biostudies-other | 2015
REPOSITORIES: biostudies-other
Schwenen Lando L G LL Hubrich Raphael R Milovanovic Dragomir D Geil Burkhard B Yang Jian J Kros Alexander A Jahn Reinhard R Steinem Claudia C
Scientific reports 20150713
Even though a number of different in vitro fusion assays have been developed to analyze protein mediated fusion, they still only partially capture the essential features of the in vivo situation. Here we established an in vitro fusion assay that mimics the fluidity and planar geometry of the cellular plasma membrane to be able to monitor fusion of single protein-containing vesicles. As a proof of concept, planar pore-spanning membranes harboring SNARE-proteins were generated on highly ordered fu ...[more]