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Dingar_in_vivo-BioID_P103_VS6

ABSTRACT: This submission is the mass spectrometry dataset for the manuscript by Dingar et al. (In vivo BioID identifies CHD8 as a novel c-Myc interacting protein). See the README file within "Methods and Protocols" for the accompanying File descriptions.

PROVIDER: MSV000078518 | MassIVE | Fri Jan 03 11:49:00 GMT 2014

REPOSITORIES: MassIVE

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BioID identifies novel c-MYC interacting partners in cultured cells and xenograft tumors.

Dingar Dharmendra D Kalkat Manpreet M Chan Pak-Kei PK Srikumar Tharan T Bailey Swneke D SD Tu William B WB Coyaud Etienne E Ponzielli Romina R Kolyar Max M Jurisica Igor I Huang Annie A Lupien Mathieu M Penn Linda Z LZ Raught Brian B

Journal of proteomics 20141018

The BioID proximity-based biotin labeling technique was recently developed for the characterization of protein-protein interaction networks [1]. To date, this method has been applied to a number of different polypeptides expressed in cultured cells. Here we report the adaptation of BioID to the identification of protein-protein interactions surrounding the c-MYC oncoprotein in human cells grown both under standard culture conditions and in mice as tumor xenografts. Notably, in vivo BioID yielded ...[more]

PMID: 25452129

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