Dyakov_etal_Nuclear_Bodies_BioID_Map_2023

ABSTRACT: This dataset consists of the files for 334 BioID samples: .wiff and .wiff.scan raw MS files, .mzML peak files, and MSPLIT results files; all acquired on TripleTOF 6600 mass spectrometers operated in Data Independent Acquisition mode (SWATH). It also includes the sequence database used for analysis and the MSPLIT spectral library which was generated from paired samples acquired in Data Dependent Acquisition (DDA) mode. All sample generation, streptavidin affinity purification, mass spectrometric acquisition, and data analysis was performed by Boris Dyakov. 13 of the stable cell lines used in this study were generated in Anne-Claude Gingras’ lab by Ji-Young Youn and Wade Dunham (Lunenfeld-Tanenbaum Research Institute); 6 of the stable cell lines were generated in Eric Shoubridge’s lab (McGill University) by Hana Antonicka. The files are associated with a manuscript submitted for publication by Dyakov et al. that provides a spatial map of nuclear body-associated proteins with a focus on the paraspeckle and nuclear speckle. Refer to the corresponding table in the published manuscript for a full description of all baits used; 36/146 baits included in this dataset were previously used in earlier publications and collaborations from our group. Anne-Claude Gingras is the corresponding author of the manuscript and should be contacted for questions about this dataset ([e-mail]).

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Anne-Claude Gingras  

PROVIDER: MSV000090684 | MassIVE | Wed Nov 09 15:36:00 GMT 2022

SECONDARY ACCESSION(S): PXD038077

REPOSITORIES: MassIVE