Proteomics

Dataset Information

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Quantitative proteomic analysis of chromatin-associated factors


ABSTRACT: Not available

INSTRUMENT(S): instrument model

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Attila Csordas  

PROVIDER: PRD000481 | Pride | 2012-07-26

REPOSITORIES: Pride

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Publications

Quantitative proteomic analysis of chromatin-associated factors.

Shiio Yuzuru Y   Eisenman Robert N RN   Yi Eugene C EC   Donohoe Sam S   Goodlett David R DR   Aebersold Ruedi R  

Journal of the American Society for Mass Spectrometry 20030701 7


A method to identify and quantify chromatin-associated proteins has been developed and applied to the analysis of changes in chromatin-associated proteins induced by Myc oncoprotein expression in human B lymphocytes. Chromatin-enriched fractions were isolated by differential detergent/salt extraction and analyzed by ICAT reagent labeling, multi-dimensional chromatography and tandem mass spectrometry. Many known chromatin-associated regulatory factors were identified and quantified. The method wi  ...[more]

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