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Plasma Proteome Profiling of CML in Pakistan

ABSTRACT: Systematic characterization of the plasma proteome in healthy and diseased state may uncover the potential biomarkers of disease diagnosis, prognosis and/or relapse detection. We, in this research project, have employed two-dimensional gel electrophoresis in conjunction with mass spectrometry-based analysis in a proteome-wide search for the identification of biomarkers of chronic-phase chronic myeloid leukemia (CP-CML).

INSTRUMENT(S): ultraflex

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Blood Plasma

DISEASE(S): Chronic Myeloid Leukemia

SUBMITTER: Muhammad Akhtar

LAB HEAD: Prof. Dr. M. Waheed Akhtar

PROVIDER: PXD002757 | Pride | 2017-01-30

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	AACT.fasta	Fasta
	CD5L.fasta	Fasta
	CPCML.png	Other
	FTTAoeEOE.dat	Other
	FTTAoeEeS.dat	Other

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Publications

CD5 molecule-like and transthyretin as putative biomarkers of chronic myeloid leukemia - an insight from the proteomic analysis of human plasma.

Fatima Iram I Sadaf Saima S Musharraf Syed Ghulam SG Hashmi Naghma N Akhtar Muhammad Waheed MW

Scientific reports 20170124

Better and sensitive biomarkers are needed to help understand the mechanism of disease onset, progression, prognosis and monitoring of the therapeutic response. Aim of this study was to identify the candidate circulating markers of chronic-phase chronic myeloid leukemia (CP-CML) manifestations, having potential to develop into predictive- or monitoring-biomarkers. A proteomic approach, two-dimensional gel electrophoresis in conjunction with mass spectrometry (2DE-MS), was employed for this purpo ...[more]

PMID: 28117336

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2021-09-08 | PXD014352 | Pride

Tissue Proteome Profiling of Ovarian cancer in Pakistani Population.

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Sex Impacts Cardiac Function and the Proteome Response to Thyroid Hormone in Aged Mice

Project description:Background: Sex and age have substantial influence on thyroid function. Sex influences the risk and clinical expression of thyroid disorders (TDs), with age a proposed trigger for the development of TDs. Cardiac function is affected by thyroid hormone levels with gender differences. Accordingly, we investigated the proteomic changes involved in sex based cardiac responses to thyroid dysfunction in elderly mice. Methods: Aged (18-20 months) male and female C57BL/6 mice were fed diets to create euthyroid, hypothyroid, or hyperthyroid states. Serial echocardiographs were performed to assess heart function. Proteomic changes in cardiac protein profiles were assessed by 2-D DIGE and LC-MS/MS, and a subset confirmed by immunoblotting. Results: Serial echocardiographs showed ventricular function remained unchanged regardless of treatment. Heart rate and size increased (hyperthyroid) or decreased (hypothyroid) independent of sex. Pairwise comparison between the six groups identified 55 proteins (≥ 1.5-fold difference and p < 0.1). Compared to same-sex controls 26/55 protein changes were in the female hypothyroid heart, whereas 15/55 protein changes were identified in the male hypothyroid, and male and female hyperthyroid heart. The proteins mapped to oxidative phosphorylation, tissue remodeling and inflammatory response pathways. Conclusion: We identified both predicted and novel proteins with gender specific differential expression in response to thyroid hormone status, providing a catalogue of proteins associated with thyroid dysfunction. Pursuit of these proteins and their involvement in cardiac function will expand our understanding of mechanisms involved in sex-based cardiac response to thyroid dysfunction.

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Proteomic analysis of plasma membrane and tonoplast from the leaves of a mangrove plant Avicennia officinalis

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Protective cellular mechanism of estrogen against kidney stone formation: A proteomics approach and functional validation

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