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Quantitative Analysis of Global Proteome in Wheat Germ

ABSTRACT: Using iTRAQ labeling and HPLC fractionation followed by high-resolution LC-MS/MS analysis, quantitative global proteome analysis was performed. In total, 6538 protein groups were identified from Triticum_aestivum, among which 2766 proteins were quantified.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Triticum Aestivum (wheat)

TISSUE(S): Plant Cell, Germ

SUBMITTER: Yuansen Hu

LAB HEAD: Yuansen Hu

PROVIDER: PXD004564 | Pride | 2016-09-29

REPOSITORIES: Pride

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Publications

Quantitative Proteomic Analysis of Wheat Seeds during Artificial Ageing and Priming Using the Isobaric Tandem Mass Tag Labeling.

Lv Yangyong Y Zhang Shuaibing S Wang Jinshui J Hu Yuansen Y

PloS one 20160915 9

Wheat (Triticum aestivum L.) is an important crop worldwide. The physiological deterioration of seeds during storage and seed priming is closely associated with germination, and thus contributes to plant growth and subsequent grain yields. In this study, wheat seeds during different stages of artificial ageing (45°C; 50% relative humidity; 98%, 50%, 20%, and 1% Germination rates) and priming (hydro-priming treatment) were subjected to proteomics analysis through a proteomic approach based on the ...[more]

PMID: 27632285

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