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Human cerebrospinal fluid proteoform profiling

ABSTRACT: . In this study, we have investigated the capacities of last-generation Ultra-High Resolution Q-Tof systems to deal with high complexity intact protein samples, have developed the tools to process the intact protein data in an existing label-free quantification pipeline, and have evaluated the approach on a cohort of patients suffering from neurodegenerative disease in a proof-of concept study. Several proteoforms have been pointed out for their capacity to distinguish Alzheimer disease patients from patients suffering from other neurodegenerative disease and have been characterized.

INSTRUMENT(S): impact II

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cerebrospinal Fluid

DISEASE(S): Alzheimer's Disease

SUBMITTER: Pierre-Olivier SCHMIT

LAB HEAD: Dr. Christophe Hirtz

PROVIDER: PXD005314 | Pride | 2018-04-05

REPOSITORIES: Pride

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			Action	DRS
	CLUS-01.xml	Xml
	CLUS-03-11.xml	Xml
	CLUS-03_12.xml	Xml
	Chrom01.xml	Xml
	Search_CLUS-01-11.xml	Xml

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Publications

Towards a routine application of Top-Down approaches for label-free discovery workflows.

Schmit Pierre-Olivier PO Vialaret Jerome J Wessels Hans J C T HJCT van Gool Alain J AJ Lehmann Sylvain S Gabelle Audrey A Wood Jason J Bern Marshall M Paape Rainer R Suckau Detlev D Kruppa Gary G Hirtz Christophe C

Journal of proteomics 20170830

Thanks to proteomics investigations, our vision of the role of different protein isoforms in the pathophysiology of diseases has largely evolved. The idea that protein biomarkers like tau, amyloid peptides, ApoE, cystatin, or neurogranin are represented in body fluids as single species is obviously over-simplified, as most proteins are present in different isoforms and subjected to numerous processing and post-translational modifications. Measuring the intact mass of proteins by MS has the advan ...[more]

PMID: 28855124

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