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BL6vsTLR7-/- label-free analysis

ABSTRACT: Analysis of proteins differentially regulated in the presence or absence of TLR7 (in murine C57BL/6-derived cells)

OTHER RELATED OMICS DATASETS IN: PRJNA406928

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Macrophage

SUBMITTER: Nathan Croft

LAB HEAD: Anthony Purcell

PROVIDER: PXD007239 | Pride | 2018-03-05

REPOSITORIES: Pride

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			Action	DRS
	P1NC20160825_cnathan_Ferrand_245_wt_NT.raw	Raw
	P1NC20160825_cnathan_Ferrand_246_wt_NT.raw	Raw
	P1NC20160825_cnathan_Ferrand_247_wt_NT.raw	Raw
	P1NC20160825_cnathan_Ferrand_248_KO_NT.raw	Raw
	P1NC20160825_cnathan_Ferrand_249_KO_NT.raw	Raw

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1 - 5 of 8

Publications

The Use of CRISPR/Cas9 Gene Editing to Confirm Congenic Contaminations in Host-Pathogen Interaction Studies.

Ferrand Jonathan J Croft Nathan P NP Pépin Geneviève G Diener Kerrilyn R KR Wu Di D Mangan Niamh E NE Pedersen John J Behlke Mark A MA Hayball John D JD Purcell Anthony W AW Ferrero Richard L RL Gantier Michael P MP

Frontiers in cellular and infection microbiology 20180319

Murine models of <i>Salmonella enterica</i> serovar Typhimurium infection are one of the commonest tools to study host-pathogen interactions during bacterial infections. Critically, the outcome of <i>S</i>. Typhimurium infection is impacted by the genetic background of the mouse strain used, with macrophages from C57BL/6 and BALB/c mice lacking the capacity to control intracellular bacterial replication. For this reason, the use of congenic strains, which mix the genetic backgrounds of naturally ...[more]

PMID: 29616197

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Project description:Murine CD3+ T-cells were immunomagnetically purified from the spleens of C57BL/6J mice and were pretreated in vitro for three days in the presence of R848 (5 μg/ml). Gene expression profile of wild type (WT) C57BL/6J TLR7-primed T-cells, was compared to unmanipulated B6 TLR7 null CD3+ Τ-cells. We used Qiagen Toll-like Receptor RT2 Profiler PCR Array kit to quantitate gene expression profiling of the TLR signaling pathway.

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