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Proteomic Analysis of rattus norvegicus colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea by label-free LC–MS/MS

ABSTRACT: To investigate the molecular pathological mechanisms of irritable bowel syndrome with diarrhea (IBS-D) and elucidate the effects of acupuncture on IBS-D colonic mucosa protein abundance in rats, a label-free high-throughput liquid chromatography-tandem mass spectrometry (LC-MS)-based proteomics analysis was used to survey the global changes of colonic mucosa proteins between different groups. A Nano flow Ultimate 3000 HPLC (Dionex Corp, Sunnyvale, CA) coupled online to a Q-Exactive Plus mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) was used in this project.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Colonic Mucosa

DISEASE(S): Irritable Bowel Syndrome

SUBMITTER: Jing Liu

LAB HEAD: Jia Li

PROVIDER: PXD025558 | Pride | 2022-10-14

REPOSITORIES: Pride

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Publications

Proteomic analysis of rat colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea.

Liu Jing J Peng Rui R Tan Qian Q Li Bocun B Chen Jianyi J Liu Guangya G Wang Yawen Y Li Chao C Li Jia J Wang Hua H

PloS one 20220912 9

To investigate the molecular pathological mechanisms of irritable bowel syndrome with diarrhea (IBS-D) and elucidate the effects of acupuncture on IBS-D colonic mucosa protein abundance in rats, a label-free high-throughput liquid chromatography-tandem mass spectrometry (LC-MS)-based proteomics analysis was used to survey the global changes of colonic mucosa proteins between different groups. Sixteen Sprague-Dawley (SD) male rats were randomly divided into four groups: the control group (C); the ...[more]

PMID: 36094925

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