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Ubiquitin-based pathway acts inside chloroplasts to regulate photosynthesis

ABSTRACT: Photosynthesis is the energetic basis for most life on Earth, and in plants it operates inside double-membrane-bound organelles called chloroplasts. The photosynthetic apparatus comprises numerous proteins encoded by the nuclear and organellar genomes. Maintenance of this apparatus requires the action of internal chloroplast proteases, but a role for the nucleocytosolic ubiquitin-proteasome system (UPS) was not expected owing to the barrier presented by the double-membrane envelope. Here, we show that photosynthesis proteins (including those encoded internally by chloroplast genes) are ubiquitinated, and processed via the CHLORAD pathway: they are degraded by the 26S proteasome following CDC48-dependent retrotranslocation to the cytosol. This demonstrates that the reach of the UPS extends to the interior of endosymbiotically-derived chloroplasts, where it acts to regulate one of the most fundamental processes of life.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Leaf

SUBMITTER: Honglin Chen

LAB HEAD: Shabaz Mohammed

PROVIDER: PXD031388 | Pride | 2022-12-06

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	6xMyc_WTchloroplast_txt.zip	Other
	PTMscan_WTchloroplast_txt.zip	Other
	QEX01_200129_ChloroplastMycUb_Beads_06.raw	Raw
	QEX01_200129_ChloroplastMycUb_Eluate_04.raw	Raw
	QEX03_210305_PTMscan_wt_IP_63.raw	Raw

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Ubiquitin-based pathway acts inside chloroplasts to regulate photosynthesis

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