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Mus musculus

ABSTRACT: Genome-wide mapping of Tbx20 (GFP-tagged) in adult mouse whole heart

PROVIDER: PRJNA141343 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR223492.fastq.gz	Fastqsanger.gz
	SRR223493.fastq.gz	Fastqsanger.gz
	SRR223494.fastq.gz	Fastqsanger.gz
	SRR223495.fastq.gz	Fastqsanger.gz

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Genome-wide mapping of Tbx20 (GFP-tagged) in adult mouse whole heart

Project description:Tbx20 is a transcription factor important for heart development. To assess the role of Tbx20 in the adult heart, we sought to identify regulatory regions, gene targets and pathways regulated by this TF. To this end, we used chromatin immunoprecipitation (ChIP) of Tbx20 fused to GFP to locate binding sites in the genome of 8 weeks whole heart mouse tissue. Analysis of 1 Tbx20-GFP ChIP sample of adult mouse whole heart against whole heart input

2011-11-21 | E-GEOD-29636 | biostudies-arrayexpress

Genome-wide mapping of Tbx20 (GFP-tagged) in adult mouse whole heart

2011-11-21 | GSE29636 | GEO

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Project description:Genome-wide profiling of gene expression of normal and Tbx20 knockout adult mouse whole heart

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Genome-wide profiling of gene expression of normal and Tbx20 knockout adult mouse whole heart

Project description:Tbx20 is a transcription factor important for heart development. To assess the role of Tbx20 in the adult heart, we generated a conditional knockout for this gene, specifically in cardiomyocytes. We profiled gene expression levels using RNA-seq in both normal and knockout adult mouse hearts to identify genes and pathways regulated by Tbx20. The article describing the Tbx20 knockout mouse is under review, a reference will be added when published.

2012-02-10 | GSE30943 | GEO

Genome-wide profiling of gene expression of normal and Tbx20 knockout adult mouse whole heart

2012-02-10 | E-GEOD-30943 | biostudies-arrayexpress

ChIP-seq of Tbx20 tagged with GFP in E11.5 mouse hearts

Project description:We performed ChIP-seq of Tbx20 tagged with GFP in mouse hearts to identify binding sites for this transcription factor. This data set is part of the study âEndocardial Tbx20 is essential for mesenchymal and myocardial cell movements required for cardiac septationâ.

2016-06-30 | E-MTAB-3967 | biostudies-arrayexpress

ChIP-seq of Tbx20 tagged with GFP in E11.5 mouse hearts

Project description:ChIP-seq of Tbx20 tagged with GFP in E11.5 mouse hearts

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RNA-seq of wild-type and Tbx20 homozygous knockout E11.5 mouse cardiomyocytes to study Tbx20's roles in embryonic and adult mouse heart function

Project description:Tbx20 is a transcription factor known to play important roles in embryonic and adult mouse heart function. Our goal in this work was to better understand the function of this gene in embryonic (E11.5) mouse cardiomyocytes that form the developing chambers, expanding our knowledge of its role in heart development. To elucidate the role of Tbx20 in mouse cardiomyocytes, we generated conditional Tbx20 knockout and compared 4 samples with 4 samples of wild-type cardiomyocytes. We found evidence of regulation of cell cycle genes by Tbx20, which are involved in proliferation. In addition, Tbx20 seems to bind and regulate an enhancer of CoupTFII in the atrium, a gene involved in atrial development.

2018-06-14 | E-MTAB-5596 | biostudies-arrayexpress

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Project description:Genome-wide occupancy profile of Tbx3 in adult mouse whole heart

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Project description:Purpose: The goals of this study is to determine the differential expression of transcriptome in cardiomyocyte-specific Tbx20 overexpressing adult heart (DTG) compared to controls (STG).

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