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Oryza sativa Japonica Group

ABSTRACT: Transcriptome profiling of various organs at different developmental stages in rice (single-end)

PROVIDER: PRJNA243371 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1213690.fastq.gz	Fastqsanger.gz
	SRR1213691.fastq.gz	Fastqsanger.gz
	SRR1213692.fastq.gz	Fastqsanger.gz
	SRR1213693.fastq.gz	Fastqsanger.gz
	SRR1213694.fastq.gz	Fastqsanger.gz

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Oryza sativa Japonica Group

Project description:Transcriptome profiling of various organs at different developmental stages in rice (paired-end)

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Project description:Phosphate starvation/sufficient rice seedling, root or shoot Pi-starvation or Pi-sufficient stresses responsible rice genes, including previously unannotated genes were identified by Illumina mRNA-seq technology. 53 million reads from Pi-starvation or Pi-sufficient root or shoot tissues were uniquely mapped to the rice genome, and these included 40574 RAP3 transcripts in root and 39748 RAP3 transcripts in shoot. We compared our mRNA-seq expression data with that from Rice 44K oligomicroarray, and about 95.5% (root) and 95.4% (shoot) transcripts supported by the array were confirmed expression both by the array and by mRNA-seq, Moreover, 11888 (root) and 11098 (shoot) RAP genes which were not supported by array, were evidenced expression with mRNA-seq. Furthermore, we discovered 8590 (root) and 8193 (shoot) previously unannotated transcripts upon Pi-starvation and/or Pi-sufficient.

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Project description:To identificate long noncoding RNAs in rice, we profiled transcriptome of various organs at different developmental stages using nondirectional paired-end RNA-seq based on poly(A) selection. Transcriptom profiling in flower buds, flowers, flag leaves and roots sampled before flowering and after flowering, milk grains and mature seeds.

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