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Real-time quantitative PCR analysis of 3T3-L1 cells treated with Interleukin-15 (IL-15)

ABSTRACT: Differentiated 3T3-L1 adipocytes were either treated 0ng/ml or 500ng/ml rmIL-15 for 24 hours (n=3/group) qPCR gene expression profilling performed in vitro using recombinant murine (rm)IL-15 treated (500ng/ml) and untreated (control) 3T3-L1 cells. Equal amounts of total RNA per subject were used in the assay.

ORGANISM(S): Mus musculus

SUBMITTER: Ali Ashkar

PROVIDER: E-GEOD-63253 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Real-time quantitative PCR analysis of 3T3-L1 cells treated with Interleukin-15 (IL-15)

Project description:Differentiated 3T3-L1 adipocytes were either treated 0ng/ml or 500ng/ml rmIL-15 for 24 hours (n=3/group)

2014-11-14 | GSE63253 | GEO

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Project description:Differentiation of 3T3-L1 cells into adipocytes involves a highly orchestrated series of events including clonal expansion, growth arrest and terminal differentiation. The mechanisms coordinating these different steps are not yet fully understood. Here we investigated whether micro (mi)RNAs play a role in this process. Microarray analysis was performed to detect miRNA expression during 3T3-L1 preadipocyte differentiation. Several miRNAs, including let-7, were up-regulated during 3T3-L1 adipogenesis. Ectopic introduction of let-7 into 3T3-L1 cells inhibited clonal expansion as well as terminal differentiation. The mRNA encoding high mobility group AT-hook 2 (HMGA2), a transcription factor that regulates growth and proliferation in other contexts, was inversely correlated with let-7 levels during 3T3-L1 cell adipogenesis, and let-7 markedly reduced HMGA2 concentrations. Knockdown of HMGA2 inhibited 3T3-L1 differentiation. These results suggest that let-7 plays an important role in adipocyte differentiation and that it does so in part by targeting HMGA2, thereby regulating the transition from clonal expansion to terminal differentiation. 3T3-L1 cells were induced to differentiation into mature adipocytes using a canonical DMI cocktail. The time point at two days after confluency of 3T3-L1 was defined as day 0. Samples were collected at day 0, day 1, day 4, and day 7. The expression of microRNAs at day 1, day 4, and day 7 was compared to that of day 0.

2010-05-18 | E-GEOD-16229 | biostudies-arrayexpress

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Importance of histone demethylation in adipogenic differentiation and function

Project description:This SuperSeries is composed of the following subset Series: GSE18598: Differentiating 3T3-L1 adipocytes, introduced with siRNA against aof2 and rfk genes, or treated with tranylcypromine GSE18599: Differentiating 3T3-L1 adipocytes, introduced with siRNA against phf21a gene Refer to individual Series

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Differentiating 3T3-L1 adipocytes, introduced with siRNA against phf21a gene

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Mus musculus

Project description:3T3-L1-NC V.S 3T3-L1-LIGHT RNA-seq

| PRJNA451383 | ENA

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