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Transcription profiling of human blood and lymphatic endothelial cells (BECs and LECs) with and without culture in order to evaluate the artefact of cell culture on the level of the transcriptome

ABSTRACT: We compared human blood and lymphatic endothelial cells (BECs and LECs) with and without culture in order to evaluate the artefact of cell culture on the level of the transcriptome.

ORGANISM(S): Homo sapiens

SUBMITTER: Ingrid Raab

PROVIDER: E-MEXP-455 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptomal comparison of human dermal lymphatic endothelial cells ex vivo and in vitro.

Wick Nikolaus N Saharinen Pipsa P Saharinen Juha J Gurnhofer Elisabeth E Steiner Carl W CW Raab Ingrid I Stokic Dejan D Giovanoli Pietro P Buchsbaum Sabine S Burchard Aurea A Thurner Stefan S Alitalo Kari K Kerjaschki Dontscho D

Physiological genomics 20070101 2

The in vivo functions of lymphatic endothelial cells depend on their microenvironment, which cannot be fully reproduced in vitro. Because of technical limitations, gene expression in uncultured, "ex vivo" lymphatic endothelial cells has not been characterized at the molecular level. We combined tissue micropreparation and direct cell isolation with DNA chip experiments to identify 159 genes differentiating human lymphatic endothelial cells from blood vascular endothelial cells ex vivo. The same ...[more]

PMID: 17234577

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Transcription profiling of lymphatic endothelial cell subtypes expressing high and low levels of podoplanin

Project description:We microprepared native mammary skin resections from healthy female donors (breast size reduction) by a combination of enzymatic and mechanical treatment. The resulting suspensions of single dermal cells were then subjected to FACSorting using antibodies against the lymphovascular marker protein podoplanin and the panendothelial protein CD31 as positive and the leukocytic protein CD45 as negative markers. We aimed at separating lymphatic vascular endothelial cells (LECs) from blood vascular endothelial cells (BECs) in order to characterize their moelcular and functional phenotypes in health and disease. We found that lymphatic endothelial cells consisted of two instead of only one cell population. Their discrimination marker was high versus low expression of podoplanin surface protein, respectively. Especially, the low-podoplanin expressors were undescribed. Thus, we screened for their transcription profile using U133A. We identified specific marker genes and finally have assigned a specific function to the novel LEC subpopulation unknown up to now. Importantly, we did not use lysates from cell culture, but from ex vivo cells. Thus, there was no treatment of cells except processing the samples on ice.

2007-07-01 | E-TABM-227 | biostudies-arrayexpress

Transcription profiling of lymphatic endothelial cells infected with Kaposi's sacoma associated herpes virus

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2009-09-30 | E-GEOD-16357 | biostudies-arrayexpress

Transcription profiling of human lymphatic endothelial cells response to Kaposi-sarcoma herpesvirus

Project description:Assessment of the effect of Kaposi-sarcoma herpesvirus upon the transcriptome of lymphatic endothelial cells and its contribution to the transcriptome of Kaposi sarcoma.

2006-10-23 | E-MEXP-561 | biostudies-arrayexpress

The profile of cellular and KSHV microRNAs in KSHV infected lymphatic endothelial cells (6 and 72 hours post infection)

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2010-05-18 | E-GEOD-17016 | biostudies-arrayexpress

Transcription profiling of human lymphatic edothelial cells transfected with the KSHV microRNA cluster

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RNAi knock down of MAFin human lymphatic endothelial cells

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2009-10-10 | E-GEOD-16356 | biostudies-arrayexpress

Transcription profiling of Kaposi sarcoma, normal skin and primary cells

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2021-07-28 | PXD015865 | Pride

ChIP-chip study aimed at the identification of the repertoire of FOXC2 binding sites affected by the loss of FOXC2 phosphorylation in primary human lymphatic endothelial cells (LECs)

Project description:Chromatin immunoprecipitation microarray (ChIP-chip) study using anti-Myc (9E10) antibodies and primary human lymphatic endothelial cells (LECs) transduced with recombinant adenoviruses expressing wild type or phosphorylation-deficient Myc-tagged FOXC2.

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Transcription profiling of human LEC cells reveals KSHV manipulates notch signaling by upregulating Dll4 and JAG1 to alter cell cycle gene expression in

Project description:Kaposi sarcoma is the most common cancer in AIDS patients and is typified by red skin lesions. The disease is caused by the KSHV virus (HHV8) and is recognizable by its distinctive red skin lesions. The lesions are KSHV infected spindle cells, most commonly the lymphatic endothelial and blood vessel endothelial cells (LEC and BEC), plus surrounding stroma. Here we examine KSHVs modulation of Notch signaling using wild-type LEC cells co-cultured with DLL4 and JAG1 expressing LEC cells. Experiment Overall Design: There are 1) n=2 of LEC control cells co-cultured with pSIN expressing LEC, 2) n=3 of LEC co-cultured with pSIN-Dll4 expressing LEC, and 3) n=3 of LEC co-cultured with pSIN-JAG1 expressing LEC.

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