Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Translation profiling of human lung fibroblasts treated or not with rapamycin to better understand the molecular mechanism behind alternative translational routes

ABSTRACT: Deregulation of translational control is frequently implicated in the establishment and maintenance of the cancer phenotype. Anti-cancer drugs targeting protein synthesis are confronted by the problem that genes can exploit alternative translational mechanisms. To better understand the molecular mechanism behind these alternative translational routes we have exploited an ex-vivo assay that follows a competitive re-recruitment of cellular mRNAs onto polysomes in cells treated or not with rapamycin.

ORGANISM(S): Homo sapiens

SUBMITTER: Joseph Curran

PROVIDER: E-TABM-205 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

An approach to analyse the specific impact of rapamycin on mRNA-ribosome association.

Genolet Raphael R Araud Tanguy T Maillard Laetitia L Jaquier-Gubler Pascale P Curran Joseph J

BMC medical genomics 20080801

<h4>Background</h4>Recent work, using both cell culture model systems and tumour derived cell lines, suggests that the differential recruitment into polysomes of mRNA populations may be sufficient to initiate and maintain tumour formation. Consequently, a major effort is underway to use high density microarray profiles to establish molecular fingerprints for cells exposed to defined drug regimes. The aim of these pharmacogenomic approaches is to provide new information on how drugs can impact on ...[more]

PMID: 18673536

Similar Datasets

Transcription profiling of mouse primary osteoblastic cells after intermittent of continuous treatment with parathyroid hormone to investigate the role of beta-arrestin2

Project description:Role of beta-arrestin2 in response to intermittent or continuous parathyroid hormone (PTH) treatment.

2009-07-20 | E-TABM-624 | biostudies-arrayexpress

Transcription profiling of wild type and delta-sba1 yeast treated with radicicol to study gene regulation by sba1

Project description:We wanted to study regulation of gene regulation by sba1 (ortholog of mammalian p23) in yeast. sba1/p23 binds to Hsp90 and acts as a co-chaperone. We have a small molecule, radicicol, an analog of geldanamycin to inhibit the binding of p23 to Hsp90. Consequently, we have analyzed the gene expression profile in 4 conditions, wt, delta-sba1; and when Hsp90 function is impaired, that is, wt and delta-sba1 treated with radicicol.

2009-10-14 | E-TABM-573 | biostudies-arrayexpress

Transcription profiling of mouse male embryos treated with DMSO or 17beta-estradiol in wild type or ERalpha knock outs to assess the toxic effects of oestrogen exposure in fetal testis

Project description:assess the toxicogenomic effets of estrogen exposure in fetal testis

2007-10-09 | E-TABM-275 | biostudies-arrayexpress

Transcription profiling of mouse for potential detrimental effects of a phytoestrogen-rich diet on male fertility

Project description:The goal of this experiment is to assess through toxicogenomic analyses the transcriptional changes induced by dietary phytoestrogens on the fetal testis during gestational exposure.

2010-08-18 | E-TABM-769 | biostudies-arrayexpress

Transcription profiling of cortical collecting duct/connecting nephron tissue from mice treated with aldosterone, canrenoate vs. controls to identify gene products regulated by aldosterone in target cells

Project description:The mineralocorticoid hormone aldosterone controls sodium reabsorption and blood pressure largely by regulating the cell-surface expression and function of the epithelial sodium channel ENaC in target kidney tubules. Part of the stimulatory effect of aldosterone on ENaC is mediated by the induction of SGK1, a kinase that interferes with the ubiquitylation of ENaC by ubiquitin-protein ligase Nedd4-2. We performed a microarray study in order to investigate which other gene products are rapidly regulated by aldosterone in target cells that participate to the control of Na+ reabsorption and K+ secretion.

2007-03-07 | E-TABM-229 | biostudies-arrayexpress

Transcription profiling of yeast wild type and yku70, esc1, sir3 or rif1 knock-outs treated with splitomycin to study telomeric sequestration of SIRs

Project description:Budding yeast telomeres and cryptic mating-type loci are anchored at the nuclear envelope, forming foci that sequester Silent information regulators (SIR factors), much as heterochromatic chromocenters in higher eukaryotes sequester HP1. Here we examine the impact of such subcompartments for regulating transcription genome-wide. We show that the efficiency of subtelomeric reporter gene repression depends not only on the strength of SIR factor recruitment by cis-acting elements, but also on the accumulation of SIRs in perinuclear foci, which result from the clustering of telomeres. To monitor the effects of disrupting this subnuclear compartment, we performed microarray analyses under conditions that eliminate telomere anchoring, while preserving SIR complex integrity. We found 60 genes reproducibly misregulated. Among those with increased expression, 22% were within 20kb of a telomere, confirming that NE anchoring helps repress natural subtelomeric genes. In contrast, loci that were down-regulated were distributed over all chromosomes. Half of this ectopic repression was SIR-complex dependent. We conclude that released SIR factors can promiscuously repress transcription at nontelomeric genes despite the presence of "anti-silencing" mechanisms. Bioinformatic analysis revealed that promoters bearing the PAC (RNA Polymerase A and C promoters) or Abf1 binding consenses are consistently down-regulated by mislocalization of SIR factors. Thus, the normal telomeric sequestration of SIRs not only favors subtelomeric repression, but prevents promiscuous effects at a distinct subset of promoters. This demonstrates that patterns of gene expression can be regulated by changing the spatial distribution of repetitive DNA sequences that bind repressive factors.

2010-01-28 | E-TABM-630 | biostudies-arrayexpress

Transcription profiling of mouse liver after miR-122 inactivation at two Zeitgeber time points, ZT0 and ZT12

Project description:Detection of transcriptome changes associated with miR-122 inactivation in mouse liver at two Zeitgeber time points, ZT0 and ZT12.

2009-06-03 | E-TABM-692 | biostudies-arrayexpress

Transcription profiling of S. cerevisiae (budding yeast) ume6 deletion strains vs wild-type in different media to determine the complement of Ume6 dependent genes during mitosis and meiosis

Project description:To determine the complement of Ume6-dependent genes expressed during mitosis and/or meiosis in budding yeast we compared wild-type and<br>ume6 deletion strains using Yeast 2.0 high density oligonucleotide microarrays (GeneChips). Samples were analysed from cells growing in rich medium with fermentable (glucose) and non-fermentable (acetate) carbon sources and from cells undergoing meiosis and spore formation in sporulation medium. Expression data were combined with data from a genome-wide Ume6 DNA binding assay and Ume6-target site prediction to identify the most likely direct target genes of Ume6.

2014-10-01 | E-TABM-192 | biostudies-arrayexpress

Transcription profiling of chicken whole eye and retinal ganglion cells (RGC) to identify RGC-specific genes

Project description:Identification of genes regulated by ATH5 in a dose-dependent manner.; Identification of retinal ganglion cells (RGC)-specific genes.

2009-01-14 | E-TABM-417 | biostudies-arrayexpress

Transcription profiling of human, mouse and rat differentiated cell sub-types leading to sperm formation

Project description:Gene Expression profiling Study of Spermatogenesis. Comparison of the differenciated cells sub-types leading to sperm formation.

2007-05-11 | E-TABM-130 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data