Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteome analysis of RPL3L -/- mice

ABSTRACT: Proteome analysis was performed to compare protein expression levels in the hearts of RPL3L WT and deficient mice.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Keiichi I Nakayama

PROVIDER: PXD034019 | JPOST Repository | Thu Mar 16 00:00:00 GMT 2023

REPOSITORIES: jPOST

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	20211203_RPL3L_heart_200ng_KO_01.raw	Raw
	20211203_RPL3L_heart_200ng_KO_02.raw	Raw
	20211203_RPL3L_heart_200ng_KO_03.raw	Raw
	20211203_RPL3L_heart_200ng_KO_04.raw	Raw
	20211203_RPL3L_heart_200ng_WT_01.raw	Raw

Items per page:

1 - 5 of 10

Similar Datasets

Transcriptoma analysis of B splenocytes of 3'RR-deficient mice and wt mice

Project description:The IgH 3' regulatory region (3'RR) controls class switch recombination and somatic hypermutation in mice. Similar numbers of B cells are found in spleen of 3'RR-deficient mice and wt mice. We compare their transcriptoma in order to find differences in their maturation status. B splenocytes from four wt mice and four 3'RR-deficient mice are investigated. Splenic B cells are purified with anti-B220-coupled beads.

2014-01-15 | E-GEOD-45230 | biostudies-arrayexpress

Transcriptoma analysis of B splenocytes of 3'RR-deficient mice and wt mice

2014-01-15 | GSE45230 | GEO

Gene expression analysis of WT Th17 versus STAT4 deficient Th17 cells from EAE mice

Project description:The purpose of this study is to compare transcriptional profiles of WT and STAT4-deficient Th17 cultured cells from mice with experimental autoimmune encephalomyelitis (EAE) using RNA sequencing. WT and STAT4 deficient splenocytes from EAE immunized mice were cultured with MOG peptide under Th17 conditions for three days, and then total RNA was extracted from CD4 T cells for sequencing. Differential gene expression was determined using the DESeq2 algorithm. These data reveal a previously unrecognized role for STAT4 in Th17 gene expression and function.

2023-03-30 | GSE227049 | GEO

Proteome of R1441C and G2019S LRRK2 knock in mice after striatal motor learning task

Project description:LRRK2 mutations are associated with both familial and sporadic forms of Parkinson’s disease (PD). Convergent evidence suggests that LRRK2 plays critical roles in regulating striatal function. Here, by using knock-in mouse lines that express the two most common LRRK2 pathogenic mutations—G2019S and R1441C—we investigated how pathogenic LRRK2 mutations altered striatal physiology. To identify the signaling pathways that underlie the motor learning deficits, specifically in the RC mice we performed six-plex tandem mass tag (TMT) quantitative mass spectrometry (MS) to compare the protein expression of either RC or GS and WT mice following the five days of the rotarod test.

2022-12-15 | PXD037003 | Pride

RNA sequencing analysis of IECs from WT and RhoB-/- mice

Project description:Purpose: RhoB regulates cell functions, such as vesicular transport, apoptosis, autophagy, DNA repair, angiogenesis, proliferation, migration and invasion. We investigated whether RhoB is involved in the pathogenesis of ulcerative colitis (UC).We performed RNA sequencing analysis in intestinal epithelial cells (IECs) from RhoB-deficient mice and WT mice to study the molecular mechanisms through which RhoB regulates goblet cell numbers and epithelial regeneration and confirmed findings in colonic organoids from RhoB-deficient mice and WT mice and siRhoB transfected SW480 cells.

2022-09-27 | GSE170994 | GEO

Transcriptome Analysis of Tendon Derived Stem Cells (TDSCs) Isolated from WT and CFTRΔF508 Mice

Project description:We performed RNA-sequencing to compare the gene expressions of TDSCs isolated from the WT and CFTRΔF508 (DF508) mice.

2018-04-03 | GSE80385 | GEO

Transcription profiling of liver from wild type mice of different ages to determine whether gene expression changes in naturally aged mice overlap with those from DNA repair deficient mice

Project description:To investigate whether and to which extent the gene expression changes in DNA repair deficient mice (i.e. Csbm/m/Xpa-/- and Ercc1-/-) overlap with those observed in a natural aged liver, we sought to compare the full mouse liver transcriptome of adult 16-, 96- and 130-week old wt C57Bl/6J mice (n=4) with that of adult 8-week old wt C57Bl/6J mice

2006-12-21 | E-MEXP-839 | biostudies-arrayexpress

Transcript profiling in splenic red pulp macrophages from SIRPa-KO mice and WT mice

Project description:To compare the splenic macrophages between SIRPα-knockout mice and WT mice, we performed a complete transcript profiling of the splenic red pulp macrophages from SIRPα-KO mice compared to WT mice using mRNA microarray as a discovery platform. SIRPα-KO mice and WT mice were kept under the same condition. Macrophages were isolated from spleen red pulp of SIRPα-KO mice and WT mice. RNA was then isolated from the same number of freshly isolated macrophages.

2016-11-22 | GSE78191 | GEO

CRYPTOCHROMES promote daily proteome homeostasis

Project description:The daily organisation of most mammalian cellular functions is attributed to circadian regulation of clock-controlled protein expression, driven by daily cycles of CRYPTOCHROME-dependent transcriptional feedback repression. To test this, we used quantitative mass spectrometry to compare wild type and CRY-deficient fibroblasts under constant conditions. In CRY-deficient cells, we found that temporal variation in protein, phosphopeptide, and K+ abundance was at least as great as wild type controls. Most strikingly. the extent of temporal variation within either genotype was much smaller than overall differences in proteome composition between WT and CRY-deficient cells. This proteome imbalance in CRY-deficient cells and tissues was associated with increased susceptibility to proteotoxic stress, which impairs circadian robustness, and may contribute to the wide-ranging phenotypes of CRY-deficient mice. Rather than generating largescale daily variation in proteome composition, we suggest it is plausible that the various transcriptional and post-translational functions of CRY proteins ultimately act to promote protein and osmotic homeostasis against daily perturbation.

2021-11-19 | PXD019499 | Pride

Proteome of WT and rss03 deletion mutant

Project description:We employed a quantitative differential proteomics approach to compare the protein expression levels between WT and rss03 deletion mutant (Δrss03), revealing significant differences in some proteins.

2024-03-15 | PXD050638 |

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data