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Snake Venom Extracellular vesicles (SVEVs) reveal wide molecular and functional proteome diversity

ABSTRACT: This study aimed at characterizing the snake venom extracellular vesicles (SVEVs).Shotgun proteomics analysis of SVEVs was performed to identify the proteome content of these vesicles. TEM, DLS, biochemical activity and cellular assays were performed to characterize the morphology and functionality of these vesicles.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Crotalus Cerberus Crotalus Viridis Agkistrodon Crotalus Atrox

TISSUE(S): Venom

SUBMITTER: Giuseppe Palmisano

LAB HEAD: Giuseppe Palmisano

PROVIDER: PXD009506 | Pride | 2022-03-07

REPOSITORIES: Pride

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Fractionation of Naja ashei venom with Size-exclusion chromatography and identification of proteins in obtained fractions

Project description:The study aimed to fractionate Naja ashei venom with Size-exclusion chromatography to obtain fractions with significantly lower complexity than crude venom. MS analysis allowed to estimate the percentage of different protein groups in each fraction, thus providing information about the extent of homogeneity in each collected sample. High purity fractions can be used to study the effects of particular proteins on different biological targets. Samples with greater complexity can be further purified using different analytical methods.

2021-09-10 | PXD023801 | Pride

Proteomic analysis of three medically important Nigerian Naja (Naja haje, Naja katiensis and Naja nigricollis) snake venoms

Project description:The venom proteomes of three medically important Nigerian Elapidae snakes Naja haje, Naja katiensis and Naja nigricollis was studied using Hydrophilic Interaction Liquid Chromatography (HILIC) coupled with LC-MS/MS analysis. Peptides/Proteins were identified and characterised using the SEQUEST and X!Tandem algorithms incorporated on to the Scaffold proteome software version 4.10.0. The Nigerian elapid species studied displayed about 70% similarity in composition of their venoms.

2021-03-12 | PXD024627 | Pride

Venom proteomic analysis of two medically important Nigerian viperidae (Echis ocellatus and Bitis arietans) snake species

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2021-03-11 | PXD024638 | Pride

Comparative gender peptidomics of Bothrops atrox venoms: new peptides and evidences of increased disintegrins levels in the venoms of females

Project description:Background: Bothrops atrox is known to be the pitviper responsible for most snakebites and human fatalities in the Amazon region. It can be found in a wide geographical area including the northern South America, the east of Andes and the Amazon basin. Possibly due to its wide distribution range and generalist feeding, intraspecific venom variation was reported by previous proteomics studies. Sex-based and ontogenetic variations on venom compositions of Bothrops snakes were also subject of proteomic and peptidomic analysis. However, the venom peptidome of B. atrox remains unknown. Methods: we conducted a mass spectrometry-based analysis of the venom peptides of individual male and female specimens combining bottom-up and top-down approaches. Results: We identified in B. atrox a total of 105 native peptides in the mass range of 0.4 to 13.9 kDa. Quantitative analysis showed that Phospholipase A2 and Bradykinin Potentiating Peptides were the most abundant peptide families in both genders, but the disintegrins levels were significantly increased in the venoms of females. Known peptides processed at non-canonical sites and new peptides were also revealed in this work. Conclusion: The venom peptidomes of male and female specimens of B. atrox were analyzed by mass spectrometry-based approaches in this work. The study points to differences in the disintegrin levels in the venoms of females that may result in distinct pathophysiology in envenomation. Further research is needed to explore its biological implications.

2020-10-13 | PXD018632 | Pride

High-throughput proteomics of the 26 medically most important elapids and vipers from sub-Saharan Africa

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2022-10-17 | PXD036161 | Pride

Exaptation of an evolutionary constraint enables behavioural control over the composition of secreted venom in giant centipedes

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2024-08-29 | PXD048264 | Pride

Distinct Transcriptional Signatures of Bone Marrow-Derived C57BL/6 and DBA/2 Dendritic Leucocytes Hosting Live Leishmania amazonensis Amastigotes

Project description:To determine the modulation of gene expression of C57BL/6 and DBA/2 BMDLs in the presence of living intracellular Leishmania amazonensis amastigotes A genome-wide transcriptional analysis was performed by comparing the gene expression profiles of control DLs and live amastigote-hosting DLs from both mouse strains. Dendritic Leucocytes were generated in vitro from bone marrow progenitors (C57Bl/6 and DBA/2 mice). Leishmania amazonensis amastigotes were purified from mouse cutaneous lesions and were added to DL cultures. After 24h, and following a sorting procedure, only BMDls housing living amastigotes were selected for total RNA extraction. Three Biological replicates per condition were run.

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2021-01-13 | PXD021561 | Pride

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