Dataset Information

Improving fractionation of human milk proteins through calcium phosphate co-precipitation and their rapid characterization by capillary electrophoresis

ABSTRACT: This work describes a simple sample pretreatment method for the fractionation of human milk proteins (into their two main groups: whey and caseins) prior to their analysis. The protein extraction protocol is based on the addition of calcium phosphate to non-adjusted pH human milk. The combination of calcium ions with phosphate results in an effective co-precipitation of caseins. To assess the suitability of this fractionation protocol, the protein extracts were analyzed by SDS-PAGE, LC-MS/MS and CE analysis. The results evidence a significant decrease in contamination of casein fraction with whey proteins and vice versa compared with the conventional isoelectric precipitation of caseins. In addition, CE fraction collection coupled to LC-MS/MS (off-line coupling) has been successfully applied to the identification of minor proteins in this complex matrix. The methodology presented here constitutes a promising tool to enlarge the knowledge of human milk proteome.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Milk

SUBMITTER: Luz Valero

LAB HEAD: Dr. José Manuel Herrero-Martínez

PROVIDER: PXD010315 | Pride | 2018-08-31

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	Fraction1_F002990.mgf	Mgf
	Fraction1_F002990.mzid.gz	Mzid
	Fraction1_F002990.pride.mgf.gz	Mgf
	Fraction1_F002990.pride.mztab.gz	Mztab
	Fraction2_F002989.mgf	Mgf

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Publications

Improving Fractionation of Human Milk Proteins through Calcium Phosphate Coprecipitation and Their Rapid Characterization by Capillary Electrophoresis.

Ten-Doménech Isabel I Simó-Alfonso Ernesto Francisco EF Herrero-Martínez José Manuel JM

Journal of proteome research 20180907 10

This work describes a simple sample pretreatment method for the fractionation of human milk proteins (into their two main groups, whey and caseins) prior to their analysis. The protein-extraction protocol is based on the addition of calcium phosphate to nonadjusted pH human milk. The combination of calcium ions with phosphate results in an effective coprecipitation of caseins. To assess the suitability of this fractionation protocol, the protein extracts were analyzed by sodium dodecyl sulfate p ...[more]

PMID: 30156851

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