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Identifying Functionally Relevant Proteins and Phosphorylation Sites During T Cell Activation Using an Integrated Thermal Shift Assay

ABSTRACT: In this study, we sought to identify how protein thermal stability integrates with changes in cell signaling and protein abundance. We developed a pipeline to investigate changes in protein abundance and thermal stabilite by combining the protein abundance and thermal shift measurements into the same TMT-plex in the Integrated Thermal Shift Assay (ITSA). Additionally, to elucidate how protein signaling corresponds to changes in thermal stability we also examined protein phosphorylation in the Phosphorylation Integrated Thermal Shift Assay (PITSA).

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): T Cell, Cytotoxic T-lymphocyte

SUBMITTER: Brandon Gassaway

LAB HEAD: Steven Gygi

PROVIDER: PXD050772 | Pride | 2024-06-17

REPOSITORIES: Pride

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			Action	DRS
	555521_ea07395_Tcell_activation_2.mzid.gz	Mzid
	555522_ea07396_Tcell_activation_2.mzid.gz	Mzid
	555523_ea07397_Tcell_activation_2.mzid.gz	Mzid
	555524_ea07398_20210815181414_Tcell_activation_2.mzid.gz	Mzid
	555525_ea07398_Tcell_activation_2.mzid.gz	Mzid

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