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Immunoprecipitation mass spectrometry of SPARKEL

ABSTRACT: SPARK is an AGC kinase found in apicomplexan parasites, which are ubiquitous pathogens of animals. Previous studies have shown that SPARK is necessary for efficient parasite calcium mobilization, invasion, and egress from host cells. We identified an interaction partner of SPARK, which we termed SPARKEL. Here, we performed immunoprecipitation of SPARKEL from parasite lysates followed by mass spectrometry to confirm the interaction with SPARK and identify other potential interacting proteins.

INSTRUMENT(S): Orbitrap Exploris 480, FAIMS compensation voltage

ORGANISM(S): Toxoplasma Gondii Gt1

SUBMITTER: Alice Herneisen

LAB HEAD: Sebastian Lourido

PROVIDER: PXD039979 | Pride | 2024-07-12

REPOSITORIES: Pride

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			Action	DRS
	20220314_TIR1_Ty_IP_DDA_real.raw	Raw
	20220314_tgALH273_308_Ty_IP_DDA_real.raw	Raw
	20220331_TIR1_Ty_IP_DDA_CV5065.raw	Raw
	20220331_tgALH273_Ty_IP_DDA_CV5065_R2.raw	Raw
	tgALH273_308_IPs_2reps_fixd.mzML	Mzml

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