Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

SPARK-mNG-mAID Immunoprecipitation Mass Spectrometry

ABSTRACT: SPARK is an AGC kinase found in apicomplexan parasites. In previous work, we showed that SPARK knockdown leads to inefficient T. gondii calcium mobilization, egress, and invasion of host cells. We performed immunoprecipitation and mass spectrometry to determine the interaction partners of SPARK-mNG-mAID isolated from T. gondii lysates. We identified a strong interaction partner, which we term SPARKEL.

INSTRUMENT(S): Orbitrap Exploris 480, FAIMS compensation voltage

ORGANISM(S): Toxoplasma Gondii Gt1

SUBMITTER: Alice Herneisen

LAB HEAD: Sebastian Lourido

PROVIDER: PXD039922 | Pride | 2024-07-12

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	20210929_TIR1_mNG_IP_ALH_DDA_4ul.raw	Raw
	20210929_TIR1_mNG_IP_TS_DDA.raw	Raw
	20210929_tgTS013_mNG_IP_ALH_DDA_4ul_20210930154811.raw	Raw
	20210929_tgTS013_mNG_IP_TS_DDA_4ul.raw	Raw
	SPARK_IPs_IPlib_2reps.mzML	Mzml

Items per page:

1 - 5 of 6

Similar Datasets

Proximity labeling of SPARKEL-TurboID

Project description:Toxoplasma gondii is a ubiquitous parasite of animals. We have previously shown that T. gondii possesses an AGC kinase, SPARK, that is necessary for calcium mobilization, invasion, and egress from host cells. Interaction studies have revealed that SPARK complexes with an elongin-like protein, which we term SPARKEL. Here, we performed proximity labeling of parasites expressing SPARKEL-TurboID endogenously.

2024-07-12 | PXD039985 | Pride

Proximity labeling of TurboID-SPARKEL

2024-07-12 | PXD039986 | Pride

PKA C3 immunoprecipitation mass spectrometry

Project description:Toxoplasma gondii possesses three protein kinase A (PKA) orthologs. PKA C3 has been implicated as a negative regulator of transformation from the acute stage tachyzoites to the chronic stage bradyzoites. We performed immunoprecipitation of PKA C3 and identified interaction partners by mass spectrometry. The apicomplexan-specific AGC kinase SPARK emerged as a strong interaction partner of PKA C3.

2024-07-12 | PXD039896 | Pride

Immunoprecipitation mass spectrometry of SPARK-mNG

Project description:SPARK is an AGC kinase found specifically in apicomplexan parasites. We have previously shown that SPARK is essential for parasite calcium mobilization, egress, and invasion. Here we have performed immunoprecipitation followed by mass spectrometry to determine interaction partners of SPARK. We identify a hypothethical protein that interacts with SPARK, which we term SPARKEL.

2024-07-12 | PXD039919 | Pride

Immunoprecipitation mass spectrometry of SPARKEL

Project description:SPARK is an AGC kinase found in apicomplexan parasites, which are ubiquitous pathogens of animals. Previous studies have shown that SPARK is necessary for efficient parasite calcium mobilization, invasion, and egress from host cells. We identified an interaction partner of SPARK, which we termed SPARKEL. Here, we performed immunoprecipitation of SPARKEL from parasite lysates followed by mass spectrometry to confirm the interaction with SPARK and identify other potential interacting proteins.

2024-07-12 | PXD039979 | Pride

Protein abundances following 24h of SPARK depletion in Toxoplasma gondii

Project description:Toxoplasma gondii is a ubiquitous eukaryotic pathogen. Apicomplexan parasites-the family to which T. gondii belongs-possess a divergent ortholog of mammalian PDK1. We have previously shown that this store potentiating/activating regulatory kinase (SPARK) is essential for T. gondii motility, invasion, and egress. Here, we conditionally depleted SPARK in live parasites for 24 hours to determine the impact of SPARK function on the parasite proteome.

2024-07-12 | PXD040598 | Pride

SPARKEL depletion time course proteome

Project description:Apicomplexan parasites are ubiquitous pathogens of humans. Toxoplasma gondii, a model apicomplexan, possesses a divergent ortholog of mammalian PDK1. This ortholog, TgSPARK, is essential for the ability of parasites to invade and exit their host. We discovered an interactor of SPARK, an elogin-like protein that we termed SPARKEL. Here, we conduct a quantitative mass spectrometry experiment to measure changes to the parasite proteome following 0, 3, 8, and 24 hours of conditional SPARKEL depletion.

2024-07-12 | PXD040635 | Pride

T. gondii SPARK depletion phosphoproteome

Project description:Apicomplexan parasites are ubiquitous pathogens of humans. Toxoplasma gondii, a model apicomplexan, possesses a divergent ortholog of mammalian PDK1. This ortholog, TgSPARK, is essential for the ability of parasites to invade and exit their host. Here, we conduct a quantitative mass spectrometry experiment to measure changes to the parasite phosphoproteome following 0, 3, 8, and 24 hours of conditional SPARK depletion.

2024-07-12 | PXD040602 | Pride

Proximity labeling of SPARK-TurboID

Project description:SPARK is an essential AGC kinase found in apicomplexan parasites, ubiquitous pathogens of humans. Previously, we showed that SPARK is necessary for parasite calcium mobilization, invasion, and egress from host cells. Here, we perform proximity labeling experiments to determine proteins sharing subcellular proximity with SPARK in live intracellular parasites.

2024-07-12 | PXD039983 | Pride

PKA C3 depletion phosphoproteomics

Project description:Toxoplasma gondii is a ubiquitous parasite of warm-blooded animals. Parasites can switch into the chronic infection following exposure to stress. PKA C3 is a protein kinase involved in the differentiation process. Here, we determine the phosphoproteome of parasites conditionally depleted of PKA C3.

2024-07-12 | PXD042989 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data