SPARK-mNG-mAID Immunoprecipitation Mass Spectrometry
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ABSTRACT: SPARK is an AGC kinase found in apicomplexan parasites. In previous work, we showed that SPARK knockdown leads to inefficient T. gondii calcium mobilization, egress, and invasion of host cells. We performed immunoprecipitation and mass spectrometry to determine the interaction partners of SPARK-mNG-mAID isolated from T. gondii lysates. We identified a strong interaction partner, which we term SPARKEL.
INSTRUMENT(S): Orbitrap Exploris 480
ORGANISM(S): Toxoplasma Gondii Gt1
SUBMITTER: Alice Herneisen
LAB HEAD: Sebastian Lourido
PROVIDER: PXD039922 | Pride | 2024-07-12
REPOSITORIES: Pride
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